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Atomic force microscopy study of the adsorption of protein molecules on transferred Langmuir monolayer
Authors:R V Ga?nutdinov  A L Tolstikhina  N D Stepina  N N Novikova  E A Yur’eva and A K Khripunov
Abstract:Ordered protein films have been obtained by the adsorption of protein molecules on a Langmuir monolayer, which had previously formed on a silicon substrate, using the Langmuir-Blodgett and molecular self-organization methods. A mixture of cholesterol with dipalmitoylphosphatidylcholine (DPPC) and a polymer—cellulose acetopivalinate—were used as immobilization materials. Protein molecules (catalase and alkaline phosphatase) immobilized on solid substrates have been investigated by atomic force micros-copy. It was shown that the developed combined technique provides a deposition of homogeneous ultrathin protein films with a high degree of filling.
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