Abstract: | Ordered protein films have been obtained by the adsorption of protein molecules on a Langmuir monolayer, which had previously
formed on a silicon substrate, using the Langmuir-Blodgett and molecular self-organization methods. A mixture of cholesterol
with dipalmitoylphosphatidylcholine (DPPC) and a polymer—cellulose acetopivalinate—were used as immobilization materials.
Protein molecules (catalase and alkaline phosphatase) immobilized on solid substrates have been investigated by atomic force
micros-copy. It was shown that the developed combined technique provides a deposition of homogeneous ultrathin protein films
with a high degree of filling. |