Analysis of single-cell differences by use of an on-chip microculture system and optical trapping |
| |
Authors: | Y Wakamoto I Inoue H Moriguchi K Yasuda |
| |
Institution: | Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Japan. |
| |
Abstract: | A method is described for continuous observation of isolated single cells that enables genetically identical cells to be compared; it uses an on-chip microculture system and optical tweezers. Photolithography is used to construct microchambers with 5-microm-high walls made of thick photoresist (SU-8) on the surface of a glass slide. These microchambers are connected by a channel through which cells are transported, by means of optical tweezers, from a cultivation microchamber to an analysis microchamber, or from the analysis microchamber to a waste microchamber. The microchambers are covered with a semi-permeable membrane to separate them from nutrient medium circulating through a "cover chamber" above. Differential analysis of isolated direct descendants of single cells showed that this system could be used to compare genetically identical cells under contamination-free conditions. It should thus help in the clarification of heterogeneous phenomena, for example unequal cell division and cell differentiation. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|