Monolithic macroporous albumin/chitosan cryogel structure: a new matrix for enzyme immobilization |
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Authors: | Martin Hedström Fatima Plieva Igor Yu Galaev Bo Mattiasson |
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Institution: | (1) Department of Biotechnology, Lund University, P.O. Box 124, 22100 Lund, Sweden |
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Abstract: | A novel monolithic macroporous material was developed by cross-linking hen egg albumin (HEA) and chitosan with glutaraldehyde
at subzero temperatures. A macroporous cryogel structure allowed efficient mass transport of solutes within the material.
In one application, albumin was partially replaced with active enzymes (glucose oxidase and horseradish peroxidase) resulting
in the production of macroporous biocatalyst preparations suitable for flow-injection analysis of glucose in the low millimolar
range. In another application, the proteolytic enzymes savinase and esperase were coupled to the macroporous structure via
free amino groups on the pore walls using glutaraldehyde as cross-linker/spacer agent. The low hydraulic resistance of the
matrix allowed for the development of a generic, high-performance online protein digestion system utilizing the wall-bound
proteases. |
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Keywords: | Chitosan Macroporous Cryogel Scaffold Staphylococcal enterotoxin B |
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