| 拟南芥叶色突变体ems3的基因定位 |
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| 引用本文： | 姜妍, 王晓萌, 杨仲南. 拟南芥叶色突变体ems3的基因定位[J]. 上海师范大学学报(自然科学版), 2009, 38(3): 287-292 |
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| 作者姓名： | 姜妍 王晓萌 杨仲南 |
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| 作者单位： | 上海师范大学,生命与环境科学学院,上海,200234 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | ems3是经甲基磺酸乙酯(EMS)诱变筛选得到的一拟南芥叶色突变体.通过背景纯化与遗传分析,发现ems3突变体是单基因隐性控制.利用图位克隆的方法对叶色基因EMS3进行了定位,结果表明:EMS3位于第5条染色体分子标记MHF15和MHF152之间55kb的区间内.生物信息预测,该区间包括有16个基因.这些结果为该基因的克隆及叶绿体发育过程中的功能研究奠定了基础.
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| 关 键 词： | 拟南芥 叶色突变体 图位克隆 分子标记 |
Genetic mapping of an Arabidopsis thaliana pigment mutant gene ems3 |
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| JIANG Yan,WANG Xiao-meng,YANG Zhong-nan. Genetic mapping of an Arabidopsis thaliana pigment mutant gene ems3[J]. Journal of Shanghai Normal University(Natural Sciences), 2009, 38(3): 287-292 |
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| Authors: | JIANG Yan WANG Xiao-meng YANG Zhong-nan |
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| Affiliation: | College of Life and Environment Sciences;Shanghai Normal University;Shanghai 200234;China |
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| Abstract: | An Arabidopsis thaliana pigment mutant ems3 was obtained by enthylmethane sulfonate(EMS)mutagenesis strategy.Genetic analysis indicated that the pigment mutant phenotype was controlled by a single recessive locus.A map-based cloning approach was used,and EMS3 was mapped to a region of 55kb between the molecular markers MHF15 and MHF152 on chromosome 5.In this region there are 16 annotated genes based on bioinformatics analysis.These results will make contributions to the molecular cloning of this gene and f... |
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| Keywords: | Arabidopsis thaliana pigment mutation mapping InDel molecular marker |
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