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原位显微红外法检测热胁迫对荒漠植物刺叶墙藓质膜透性的影响
引用本文:许书军,陈蔚红,陈颖雯,何明,王艳.原位显微红外法检测热胁迫对荒漠植物刺叶墙藓质膜透性的影响[J].光谱学与光谱分析,2007,27(12):2417-2421.
作者姓名:许书军  陈蔚红  陈颖雯  何明  王艳
作者单位:1. 上海交通大学环境科学与工程学院, 上海 200240
2. 中国科学院上海有机化学研究所,上海 200032
3. 上海交通大学农业与生物学院,上海 201101
摘    要:利用显微红外光谱法研究了不同生境下的刺叶墙藓质膜结构及其热胁迫耐性机理。从酯酰基、碳氢化合物红外吸收区域分析表明,刺叶墙藓野生叶片通过加快组织内部碳氢化合物合成速度,减少质膜渗透性,减轻高温对质膜胁迫损伤;室内培养的原丝体和新叶不能及时对热胁迫产生影响。

关 键 词:耐热性  显微红外  质膜透性  荒漠苔藓  
文章编号:1000-0593(2007)12-2417-05
收稿时间:2006-05-16
修稿时间:2006-08-26

Effects of Thermostress on the Plasma Membrane Permeability of Desert Moss Tortula desertorum Examined by In-Situ Micro-FTIR Analysis
XU Shu-jun,CHEN Wei-hong,CHEN Ying-wen,HE Ming,WANG Yan.Effects of Thermostress on the Plasma Membrane Permeability of Desert Moss Tortula desertorum Examined by In-Situ Micro-FTIR Analysis[J].Spectroscopy and Spectral Analysis,2007,27(12):2417-2421.
Authors:XU Shu-jun  CHEN Wei-hong  CHEN Ying-wen  HE Ming  WANG Yan
Institution:1. School of Enviroment Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China2. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China3. Schools of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101, China
Abstract:A technique based on Fourier transform infrared microscope (FT-IRM) was developed to detect the corresponding changes in chemical composition associated with thermostress among aging tissues of the desert moss Tortula desertorum. The results indicated that wild leaves could accelerate the rate of carbohydrate synthesis and decrease the permeability of plasma membrane during heating at 45 degrees C for 1 h. Those biological responses can alleviate the damage of heat stress, so called thermotolerance. And the ability has a positive relation to the leaf age among various wild leaves. However, the secondary protonema and leaves, cultivated in artificial incubator, were unable to adapt the change of temperature immediately and the permeability of cellular membranes was increased.
Keywords:Thermotolerance  Micro-FTIR  Protoplasm membrane permeability  Desert moss
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