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Evaluation of separation quality in two-dimensional hyphenated chromatography |
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| Authors: | Fan Gong Yi-Zeng Liang Qing-Song Xu Foo-Tim Chau King-Man Ng |
| Affiliation: | a College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China b Department of Applied Mathematics, Hunan University, Changsha 410082, PR China c Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, PR China |
| Abstract: | In this paper, a novel criterion named the overlap index (OVI) has been proposed to evaluate the separation quality in two-dimensional hyphenated chromatography. This criterion, which is obtained from the maximum value of the Gram determinant constructed by the key spectra, can reveal the degree of two-dimensional hyphenated chromatographic separation. The key spectra are selected based on the so-called D-optimal criterion. According to its geometrical sense meaning the volume spanned by the constructed vectors, OVI criterion can practically reflect the overlap degree of chromatographic peaks. The OVI criterion is further interpreted by comparing the resolution of two-dimensional data into pure chromatograms and spectra. The successful application of this OVI criterion to both of simulated two-dimensional hyphenated data and the real case in analyzing the six reference phenolic compounds from Ginkgo biloba L-leaves shows its reliability and practicality. |
| Keywords: | Hyphenated chromatography Separation quality Overlap index D-optimum |
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