肉苁蓉茎水溶性多糖SPA的结构分析

肉苁蓉为稀有的名贵中药材 ,具有补肾、益精、润肠及抗衰老等功效 [1] .研究表明 ,肉苁蓉多糖具有延缓皮肤衰老、增强机体免疫功能、促进人体外成纤维细胞的生长及促进创伤愈合 [2 ] 等生理活性作用 .对于肉苁蓉多糖的深入研究尚未见报道 ,为探讨多糖的生物活性与结构的关系 ,本文对肉苁蓉茎水溶性多糖 SPA组分进行结构分析 .有关肉苁蓉茎水溶性多糖 SPA组分的分离纯化过程见文献 [3].1　实验部分1 .1　试剂与仪器　 Sephadex G- 75 ( Pharmacia公司 ) ;二甲基亚砜 (江苏洪声化工厂 ) ;Shimadzu高压液相色谱 (日本岛津 ) ;Vavian 340 0…

Structural Analysis of Water-soluble Polysaccharide SPA Isolated from the Stem of the Cistanche Deserticola Ma

A water-soluble crude polysaccharide was isolated from the stem of the wild Cistanche deserticola Ma. It was deproteinized by Sevage method and proteinase method, and fractionated by ultrafiltration and Sephadex G-75 column chromatography. A polysaccharide fraction SPA was collected. The polysaccharide of SPA consisted of Rha, Ara, Man, Gal and Glc in the molar ratio of 1:1.83:3.06:4.56:14.74, and the average molecular weight of SPA was around 0.76×104. By means of partial hydrolysis, Smith degradation, mathylation analysis, GC-MSIR and ¹³C NMR, the results revealed the main chain was made up of(1→6)-linked-Glc residues and substituted at 3-O. On an average, there was one branch among seven main chain residues. The side chains is composed of(1→4)-linked-Gal,(1→4)-linked-Man,(1→3)-linked-Gal and(1→2)-linked-Rha residues. The nonreduced end is composed of(1→)Ara.