| 四溴双酚-A与牛血清白蛋白相互作用的光谱学及电化学研究 |
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| 引用本文： | 李淑娟,元永波,刘利军. 四溴双酚-A与牛血清白蛋白相互作用的光谱学及电化学研究[J]. 分析测试学报, 2010, 29(11). DOI: 10.3969/j.issn.1004-4957.2010.11.009 |
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| 作者姓名： | 李淑娟 元永波 刘利军 |
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| 作者单位： | 宁夏大学,化学化工学院,宁夏,银川,750021;宁夏大学,化学化工学院,宁夏,银川,750021;宁夏大学,化学化工学院,宁夏,银川,750021 |
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| 基金项目： | 宁夏自然科学基金资助项目 |
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| 摘 要： | 采用荧光光谱、同步荧光光谱、三维光谱和循环伏安曲线法研究了溴化阻燃剂四溴双酚-A(TBBPA)与牛血清白蛋白(BSA)的相互作用。研究表明,TBBPA对BSA的内源荧光有显著的猝灭作用,根据不同温度下TBBPA对BSA的荧光猝灭作用及室温的循环伏安曲线,证实猝灭机理为静态猝灭。运用位点模型计算出结合常数KA和结合位点数n,说明TBBPA与BSA仅有1个结合位点。由ΔH0和ΔS0分别为-33.06 kJ/mol和-64.73 J/(mol.K),推断范德华力或氢键在二者结合过程中起主要作用。并通过同步荧光光谱和三维光谱研究了TBBPA对BSA构象的影响,结果表明,TBBPA分子的引入改变了BSA疏水腔内疏水微环境,从而导致BSA的构象发生变化。
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| 关 键 词： | 四溴双酚-A 牛血清白蛋白 光谱 循环伏安曲线 |
Study on the Interaction of Tetrabromobisphenol A with Bovine Serum Albumin by Spectroscopy and Electrochemistry |
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| LI Shu-juan,YUAN Yong-bo,LIU Li-jun. Study on the Interaction of Tetrabromobisphenol A with Bovine Serum Albumin by Spectroscopy and Electrochemistry[J]. Journal of Instrumental Analysis, 2010, 29(11). DOI: 10.3969/j.issn.1004-4957.2010.11.009 |
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| Authors: | LI Shu-juan YUAN Yong-bo LIU Li-jun |
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| Affiliation: | LI Shu-juan,YUAN Yong-bo,LIU Li-jun(College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021,China) |
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| Abstract: | The interaction of brominated flame retardant tetrabromobisphenol A(TBBPA) and bovine serum albumin(BSA) was investigated mainly by means of fluorescence quenching spectrometry,synchronous fluorescence spectrometry,three-dimensional fluorescence spectrometry and cyclic voltammetry(CV).The results indicated that BSA was significantly quenched in the presence of TBBPA.According to the quenching effect of TBBPA on the fluorescence intensity of BSA under different temperatures and the CVs at the room temperatur... |
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| Keywords: | tetrabromobisphenol A(TBBPA) bovine serum albumin(BSA) spectroscopy cyclic voltammogram(CV) |
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