Simultaneous determination of seven phenolic acids in three Salvia species by capillary zone electrophoresis with β‐cyclodextrin as modifier |
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Authors: | Jiliang Cao Jinchao Wei Ke Tian Huanxing Su Jianbo Wan Peng Li |
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Institution: | 1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, , Macau, China;2. Center for Intestinal and Liver Inflammation Research, Stanley Manne Children's Research Institute, Feinberg School of Medicine, Northwestern University, , Chicago, IL, USA |
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Abstract: | A capillary zone electrophoresis method was developed for the simultaneous determination of seven phenolic acids, including protocatechuic aldehyde ( 1 ), salvianolic acid C ( 2 ), rosmarinic acid ( 3 ), salvianolic acid A ( 4 ), danshensu ( 5 ), salvianolic acid B ( 6 ), and protocatechuic acid ( 7 ), in Danshen and related medicinal plants. A running buffer composed of 20 mM sodium tetraborate adjusted to pH 9.0, and containing 12 mM β‐cyclodextrin as modifier. Baseline separation was achieved within 17 min running at the voltage of 20 kV, temperature of 25°C and detection wavelength of 280 nm. The relative standard deviations of migration time ranged from 0.2 to 0.7% and the peak area ranged from 1.5 to 3.7% for the seven analytes, indicating the good repeatability of the proposed method. The method was extensively validated by evaluating the linearity (R2 ≥ 0.9992), limits of detection (0.14–0.36 μg/mL), limits of quantification (0.47–1.19 μg/mL), and recovery (96.0–102.6%). Under the optimum conditions, samples of Danshen and related medicinal plants were analyzed using the developed method with high separation efficiency. |
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Keywords: | Capillary zone electrophoresis β ‐cyclodextrin Danshen Phenolic acids |
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