Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE‐Cys column |
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| Authors: | Jie Wei Aijin Shen Huihui Wan Jingyu Yan Bingcheng Yang Feifang Zhang Xinmiao Liang |
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| Affiliation: | 1. School of Pharmacy, East China University of Science and Technology, Shanghai, China;2. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China;3. Chemistry Analysis and Research Center, Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, China |
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| Abstract: | Hydrophilic interaction liquid chromatography has emerged as a valuable alternative approach to ion‐pair chromatography for the separation of aminoglycoside antibiotics in recent years. However, the resolution of structurally related aminoglycosides is a great challenge owing to the limited selectivity. In this work, a cysteine‐based zwitterionic stationary phase (named Click TE‐Cys) was utilized and compared with five commonly used hydrophilic interaction liquid chromatography columns. Click TE‐Cys displayed much better selectivity for structurally similar aminoglycosides. The retention behaviors of aminoglycosides were investigated in detail, revealing that low pH (2.7 or 3.0) and high buffer concentration (≥50 mM) were preferable for achieving good peak shape and selectivity. Effective resolution of ten aminoglycosides including spectinomycin, dihydrostreptomycin, streptomycin, gentamicin C1, gentamicin C2/C2a, gentamicin C1a, kanamycin, paromonycin, tobramycin, and neomycin was realized at optimized conditions. Additionally, spectinomycin and its related impurities were successfully resolved. The results indicated the great potential of the Click TE‐Cys column in the separation of aminoglycoside mixtures and related impurities. |
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| Keywords: | Aminoglycosides Hydrophilic interaction chromatography Separation selectivity Zwitterionic stationary phases |
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