Bovine lactoferrin purification from whey using Yellow HE‐4R as the chromatographic affinity ligand |
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Authors: | María Fernanda Baieli Nicolás Urtasun María Victoria Miranda Osvaldo Cascone Federico Javier Wolman |
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Affiliation: | Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina |
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Abstract: | The worldwide production of whey increases by around 186 million tons each year and it is generally considered as a waste, even when several whey proteins have important economic relevance. For its valorization, inexpensive ligands and integrated chromatography methods need to be developed for specific and low‐cost protein purification. Here, we describe a novel affinity process with the dye Yellow HE‐4R immobilized on Sepharose for bovine lactoferrin purification. This approach based on a low‐cost ligand showed an efficient performance for the recovery and purification of bovine lactoferrin directly from whey, with a yield of 71% and a purification factor of 61. |
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Keywords: | Bovine lactoferrin Dye affinity chromatography Sweet whey Triazine dyes |
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