Determination of naproxen in human plasma by GC–MS |
| |
Authors: | Bilal Yilmaz Huseyin Sahin Ali Fuat Erdem |
| |
Affiliation: | 1. Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey;2. Department of Emergency Medicine, Faculty of Medicine, Ataturk University, Erzurum, Turkey;3. Department of Anesthesiology and Reanimation, Faculty of Medicine, Sakarya University, Sakarya, Turkey |
| |
Abstract: | This paper describes a GC–MS method for the determination of naproxen in human plasma. Naproxen and internal standard ibuprofen were extracted from plasma using a liquid–liquid extraction method. Derivatization was carried out using N‐methyl‐N‐(trimethylsilyl)trifluoroacetamide. The calibration curve was linear between the concentration range of 0.10–5.0 μg/mL. Intra‐ and interday precision values for naproxen in plasma were <5.14, and accuracy (relative error) was better than 4.67%. The extraction recoveries of naproxen from human plasma were between 93.0 and 98.9%. The LOD and LOQ of naproxen were 0.03 and 0.10 μg/mL, respectively. Also, this assay was applied to determine the pharmacokinetic parameters of naproxen in six healthy Turkish volunteers who had been given 220 mg naproxen. |
| |
Keywords: | Ibuprofen Liquid– liquid extraction Naproxen Pharmacokinetics Validation |
|
|