Rhod-5N as a Fluorescent Molecular Sensor of Cadmium(II) Ion |
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| Authors: | Mathieu Soibinet Vincent Souchon Isabelle Leray Bernard Valeur |
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| Affiliation: | (1) Laboratoire de Chimie Générale, Conservatoire National des Arts et Métiers, 292 rue Saint-Martin, F-75141 Paris Cedex 03, France;(2) Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires, école Normale Supérieure de Cachan, 61 Avenue du Président Wilson, F-94235 Cachan Cedex, France |
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| Abstract: | The photophysical and complexing properties of Rhod-5N (commercially available) in MOPS buffer are reported. This fluorescent molecular sensor consists of a BAPTA chelating moiety bound to a rhodamine fluorophore. Its fluorescence quantum yield is low and a drastic enhancement of fluorescence intensity upon cation binding was observed. Special attention was paid to the complexation with Cd2+, a well known toxic metal ion. Possible interference with other metal ions (Na+, K+, Mg2+, Ca2+, Zn2+, Pb2+) was examined. Rhod-5N was found to be highly selective of Cd2+ over those interfering cations except Pb2+. The limit of detection is 3.1 μg l−1. |
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| Keywords: | Fluorescence Cadmium Sensing Fluorescent molecular sensors |
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