双马来腈二亚胺合铂与DNA的作用

本文以紫外-可见光谱、荧光光谱及黏度法研究了双马来腈二亚胺合铂与DNA的作用。紫外-可见光谱的研究表明，与DNA作用后，双马来腈二亚胺合铂在可见区的吸收显示出了减色效应，并伴随着吸收峰的蓝移。Scatchard图的分析结果表明，双马来腈二亚胺合铂与DNA的作用位点与溴化乙锭不同。黏度法实验表明，双马来腈二亚胺合铂与DNA作用后降低了DNA的相对黏度。这些研究结果表明，双马来腈二亚胺合铂以静电作用方式与DNA结合。本研究有助于深入理解双马来腈二亚胺合铂的作用机理并开发这种潜在的新型光动力治疗剂。

Interaction of DNA with Bis(diiminosuccinonitrilo)platinum(II)

Interaction of DNA with bis(diiminosuccinonitrilo)platinum(II) has been studied by UV‐visible absorbance spectra, fluorescence spectra and viscosity measurements. The UV‐visible absorption spectra of the metal complex exhibit hypochromism with a small blue shift on interaction with DNA. Scatchard plot analyses indicate that the binding sites of the metal complex on DNA are different from those of ethidium bromide. Viscosity experiments reveal that the binding of the metal complex decreases the relative viscosity of DNA. These results suggest that the platinum diimine complex interact with DNA by surface binding. These studies are helpful for us to understand the action mechanism ofbis(diiminosuccinonitrilo)platinum(II) as a potential photodynamic therapeutic agent, and further to develop it.