Proteomic analysis of the cellular proteins induced by adaptive concentrations of hydrogen peroxide in human U937 cells |
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Authors: | Seong Je Kyung Kim Do Kyun Choi Kun Ho Oh Seung Hyun Kim Kil Soo Lee Seung-Sook Um Hong-Duck |
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Institution: | Laboratory of Developmental Biology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea. |
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Abstract: | When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this study, the total proteins extracted from human U937 leukemia cells exposed to 50 micromM H(2)O(2) for 24 h to induce an optimal protective response were analyzed by two-dimensional polyacrylamide gel electrophoresis. H(2)O(2) treatment induced elevation of level of 34 protein spots. An analysis of these spots by a matrix associated laser desorption/ionization time-of-flight mass spectrometry identified 28 of the H(2)O(2)-induced proteins. These include proteins involved in energy metabolism, translation and RNA processing, chaperoning or mediating protein folding, cellular signaling, and redox regulation, as well as a mitochondrial channel component, and an actin-bundling protein. Therefore, it appears that the cellular adaptation to oxidative stress is a complex process, and is accompanied by a modulation of diverse cellular functions. |
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