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The effect of point mutation on the equilibrium structural fluctuations of ferric Myoglobin
Authors:Adamczyk Katrin  Candelaresi Marco  Kania Rafal  Robb Kirsty  Bellota-Antón Cesar  Greetham Gregory M  Pollard Mark R  Towrie Michael  Parker Anthony W  Hoskisson Paul A  Tucker Nicholas P  Hunt Neil T
Institution:Department of Physics, University of Strathclyde, Glasgow, Scotland, UK. katrin.adamczyk@strath.ac.uk
Abstract:The ultrafast equilibrium fluctuations of the Fe(III)-NO complex of a single point mutation of Myoglobin (H64Q) have been studied using Fourier Transform 2D-IR spectroscopy. Comparison with data from wild type Myoglobin (wt-Mb) shows the presence of two conformational substates of the mutant haem pocket where only one exists in the wild type form. One of the substates of the mutant exhibits an almost identical NO stretching frequency and spectral diffusion dynamics to wt-Mb while the other is distinctly different in both respects. The remarkably contrasting dynamics are largely attributable to interactions between the NO ligand and a nearby distal side chain which provides a basis for understanding the roles of these side chains in other ferric haem proteins.
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