Method development and validation for the simultaneous determination of four coumarins in Saussurea superba by capillary zone electrophoresis |
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Authors: | Chen Juan Chen Li Zhang Hong-Li Shi Yan-Ping |
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Institution: | Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou 730000, People's Republic of China. |
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Abstract: | A capillary zone electrophoretic method has been developed for the determination of four coumarins--skimmin, scopolin, scopoletin, and umbelliferone-in Saussurea superba with UV detection at 254 nm. The capillary temperature was kept constant at 25 degrees C. Effects of buffer pH, electrolyte concentration, organic modifier, and applied voltage on migration behavior were studied systematically. The optimum conditions for separation were achieved by using 30 mM borate buffer at pH 9.02 containing 15% (v/v) methanol as the electrolyte and 25 kV as the applied voltage. For all analytes a good linear regression relationship (r > 0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, and accuracy. The validated method was successfully applied to the simultaneous determination of the four analytes in S. superba. |
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