Quantitative analysis of closantel and rafoxanide in bovine and ovine muscles by high-performance liquid chromatography with fluorescence detection |
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Authors: | Yeung Hoi-Sze Ching Wing-Hong Lai Shirley Sau-Ling Lee Wai-On Wong Yiu-Tung |
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Institution: | Analytical and Advisory Services Division, Government Laboratory, 7/F Ho Man Tin Government Office, Ho Man Tin, Kowloon, Hong Kong Special Administrative Region, China. |
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Abstract: | An HPLC method with a fluorescence detector (HPLC-FLD) was described for the quantitative determination of closantel and rafoxanide in bovine and ovine muscles. A structural analog closely related to rafoxanide, viz., N-4-(4-chlorophenoxy)phenyl]-2-hydroxy-3,5-diiodobenzamide, was synthesized as an internal standard. Bovine and ovine muscles were extracted with acetonitrile-acetone (60 + 40, v/v) followed by cleanup on mixed mode anionic exchange SPE cartridges. After evaporation and reconstitution with the mobile phase, the sample was analyzed by HPLC-FLD using internal standard calibration. The method was validated by using fortified bovine and ovine muscles at 15, 30, and 60 microg/kg. The accuracy and RSD were 70-110% and < or =10%, respectively. |
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