Transport of acetylcholine in a membrane |
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Authors: | Sachio Hirose Wolf R Vieth |
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Institution: | (1) Central Research Laboratories, Mitsubishi Petrochemical Co., Ltd., 1315, Ami, Inashiki, 3003 Ibaraki, Japan;(2) Department of Chemical and Biochemical Engineering, Rutgers University, 08854 Piscataway, NJ |
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Abstract: | A laminate model of the cleft-plus-postsynaptic membrane structure of the neuromuscular junction was studied. In order to
prepare a model of the postsynaptic membrane, the properties of acetylcholine (Ach) receptor-rich vesicles purified from Torpedo
fish were measured. Immobilization of vesicles was demonstrated by various methods, in particular, by investigating collagen
and carrageenan matrices as models of the fluidfilled fibrous matrix of the cleft. It was found that a laminated system employing
a liquid membrane-containing vesicle suspension, together with a swollen collagen membrane, is an appropriate model for examining
important transport/reception aspects of the cleft-plus-postsynaptic membrane structure.
Combined transport with immobilization of Ach in the liquid membrane system was elucidated and effective diffusivities in
the vesicle suspension layer were calculated. Effective diffusivities of the composite system simulating the cleft and the
postsynaptic membrane were evaluated as well. These data illustrate the importance of penetrant immobilization in retarding
the diffusion process during neurotransmission. |
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Keywords: | Transport of acetylcholine in a membrane acetylcholine trans-port in a membrane membrane acetylcholine transport in |
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