Displacement and spacer displacement chromatography. Alteration of spot profile in displacement thin-layer chromatography |
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Authors: | H. Kalász Mária Báthori J. Nagy |
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Affiliation: | (1) Department of Pharmacology, Semmelweis University of Medicine, Budapest, Hungary;(2) Department of Pharmacognosy, Szent-Györgyi University of Medicine, Szeged, Hungary |
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Abstract: | Summary Migration of spots in displacement development was investigated using a planar stationary phase. The effects of the presence or absence of the additive substances in the carrier were demonstrated when these compounds also played a role in the competition for the binding sites of the stationary phase; however, they were not members of the displacement train.In the case of displacement chromatography, compounds A+B are well separated but located very closely to each other. Using a spacer (C) in the sample, the peaks or spots of A and B are aparted by C and their virtual separation is increased.When compounds A+B were displaced by each other as well as by the displacer, the presence of the additive compound C in the carrier (carrier-spacer) can transfer an otherwise displaced component (A) into the so-called overloaded elution region of the chromatogram. At the same time the additive compound C does not unconditionally reduce the performance of displacement chromatography.Presented at the 17th International Symposium on Chromatography, September 25–30, 1988, Vienna, Austria. |
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Keywords: | Displacement chromatography Displacement TLC Overloaded elution chromatography Spacer displacement thin-layer chromatography Spot profile |
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