Amperometric assay for collagenase |
| |
| Authors: | That T Ngo Howard M Lenhoff |
| |
| Institution: | (1) Department of Developmental and Cell Biology, University of California, 92717 Irvine, CA;(2) Department of Molecular Biology and Biochemistry, University of California, 92717 Irvine, CA |
| |
| Abstract: | A sensitive and rapid amperometric assay for collagenase has been developed. The substrate for the assay is glucose oxidase
covalently linked to insoluble collagen with dimethylsuberimidate. The collagenase cleaves the insoluble collagen-glucose
oxidase conjugate into smaller, soluble fragments that have glucose oxidase activity. That activity is proportional to the
collagenase activity hydrolyzing the insoluble conjugate. In the absence of collagenase, no glucose oxidase activity is found
in the soluble phase. Glucose oxidase activity was assayed by measuring amperometrically the rate at which hydrogen peroxide
is produced. The kinetics follow that proposed for a soluble enzyme acting on an insoluble substrate. |
| |
| Keywords: | Amperometric assay for collagenase assay amperometric for collagenase collagenase amperometric assay for glucose oxidase in amperometric assay for collagenase collagen amperometric assay for collagenase |
| 本文献已被 SpringerLink 等数据库收录！ |
