硼酸及硼酸酯类过氧化氢荧光探针的最新研究进展

生物新陈代谢过程中产生的过氧化氢（H2O2）是生命活动所必需的,但是过量过氧化氢的存在可以引发多种疾病,因此对体内过氧化氢的检测具有重要意义.采用荧光探针法,借助激光共聚焦成像技术能够实现对活细胞和组织内的过氧化氢＂实时、可见、定量＂的检测,为深入阐明过氧化氢在生理和病理过程中所起的作用提供了一个重要手段.本文按荧光探针的结构分类,对近几年来以硼酸及硼酸酯基团作为荧光开关的具有高选择性和灵敏度的过氧化氢荧光探针进行了综述,主要探讨其设计思想、作用机制及应用,为过氧化氢探针的设计提供了新思路.

Recent advances in the boric acid/boronate-based fluorescent probes for detection of hydrogen peroxide

The physiological level of hydrogen peroxide（H2O2） is believed to be indispensable in regulating diverse cellular processes.However,excessive hydrogen peroxide generation is involved in the pathogenesis of many diseases,including cardiovascular disease,cancer,and neurological disorders.Thus,it is of significance to detect hydrogen peroxide in vivo.Fluorescent probe method,combined with confocal laser scanning microscopy（CLSM） can be used in real-time,visible and quantitative detection of hydrogen peroxide in living cells and tissues,which provides powerful tools for the understanding of hydrogen peroxide in physiological and pathological processes.This critical review highlights recent advances of design,mechanisms and applications of boric acid/boronate-based fluorescent probes employed to monitor hydrogen peroxide.