液相色谱串联质谱法研究小刺猴头菌子实体中污染物增塑剂

采用GC/MS和LC-MS/MS联用技术对栽培小刺猴头菌子实体中分离得到的组分A进行分析, 检测出邻苯二甲酸二正辛酯(DNOP)和邻苯二甲酸二丁酯(DBP)两种增塑剂, 建立了对有可能造成栽培食用菌被污染的9种常见增塑剂的同时检测方法, 该方法的回收率为71.6%~82.2%, 精密度为2.11%~8.53%, 检测限为0.002~0.030 μg/L

Determination of Nine Plasticizers in the Body of Hericium Caput-Medusaeby Liquid Chromatography-Tandem Mass Spectrometry

A composition from the cultured body of Hericium caput-medusae(Bull.: Fr.) Pers.] was analyzed quantitatively and qualitatively by gas chromatography-mass spectrometry(GC-MS) and liquid chromatography-tandem mass spectrometry(LC-MS/MS). Di-n-octyl o-phthalate(DOP) and di-n-butyl o-phthalate(DBP)were identified by manually mass spectrum analyzing and mass spectrum bank matching. Since plastics are not inert, the plasticizers might migrate into the cultured mushroom and cause contamination. A simple LC-MS/MS method was established for the determination of nine plasticizers in cultured edible mushroom due to potential contamination. The method shows a good recovery(71.6%—82.2%) for various levels of spiked samples(0.01—0.10 mg/L).The relative standard deviations were in the range of 2.11%—8.53% for all plasticizers studied. The limits of detection were in the range of 0.002—0.30 μg/L.