Electron transfer properties and electrocatalytic behavior of tyrosinase on ZnO nanorod |
| |
Authors: | Liyuan Chen Baoxiang Gu Guoping Zhu Yafeng Wu Songqin Liu Chunxiang Xu |
| |
Institution: | aState Key Laboratory of Bioelectronics and Jiangsu Provincial Key Laboratory of Biomaterials and Biodevices, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China;bAdvanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Najing 210096, PR China |
| |
Abstract: | In this work, the adsorption of tyrosinase on ZnO nanorods and its electrocatalytic behaviors were investigated. The mushroom tyrosinase with low isoelectric point was expected to adhere on the positively charged surface of ZnO nanorods by electrostatic attraction in a neutral solution. Scanning electron microscope images and spectroscopic analysis demonstrated the adsorption of tyrosinase on ZnO nanorods and the adsorbed tyrosinase remain its bioactivity to a large extent. In the presence of tyrosinase, a roughly and cyathiform of nanosized ZnO films was obtained. This open, three-dimensioned ramiform structure made the move through and exchange the electron with GCE more easily, and thus accelerating the electron transfer between electroactive and GCE. The adsorbed tyrosinase could catalyze the oxidation of phenol and catechol. The linear concentration ranges were from 0.02 to 0.1 mM and 0.01 to 0.4 mM, for phenol and catechol, respectively. The apparent Michaelis-menten constant , a reflection of the enzymatic affinity, was 0.24 mM for phenol and 1.75 mM for catechol, which suggests a large affinity to phenolic compound. The proposed methods presented a way for further studies of the immobilization and electrochemistry of proteins on nanostructured materials. |
| |
Keywords: | ZnO nanorods Tyrosinase Phenolic compound Biosensor |
本文献已被 ScienceDirect 等数据库收录! |
|