Tunneling and coupled motion in the Escherichia coli dihydrofolate reductase catalysis |
| |
Authors: | Sikorski R Steven Wang Lin Markham Kelli A Rajagopalan P T Ravi Benkovic Stephen J Kohen Amnon |
| |
Affiliation: | Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, USA. |
| |
Abstract: | H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1 degrees to 2 degrees coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|