三七总皂苷对新生大鼠海马神经干细胞活性影响及促分化作用

目的研究三七总皂苷对海马神经干细胞活性的影响和分化作用。方法体外培养海马神经干细胞,分别接种于96孔板和12孔板,96孔板细胞按三七总皂苷不同浓度梯度和同一浓度的不同时间点进行干预,应用MTT法检测海马神经干细胞的OD值,观察三七总皂苷对海马神经干细胞活性的影响;12细胞孔板分为对照组和给药组,应用免疫荧光染色方法检测神经元新生特异抗原（Tuj-1）和胶质细胞新生抗原（Vimentin）的表达,以观察三七总皂苷对海马神经干细胞分化的影响。结果（1）一定浓度范围内三七总皂苷能增强海马神经干细胞活性;（2）三七总皂苷能促进海马神经干细胞向神经元和胶质细胞方向分化。结论三七总皂苷能增强海马神经干细胞的活性并能促进海马神经干细胞分化。

Effects of panax notoginseng saponing on activity and differentiation of neonate rat hippocampus neural stem cells in vitro

Objective: Study the effects of panax notoginseng saponing on activity and differentiation of hippocampus neural stem cells in vitro.Methods: Hippocampus neural stem cells cultured in vitro differently were seeded in 96 plate and 12 plate,and the cells cultured in 96 plate were intervented with diversity concentration panax notoginseng saponing and at different time with same concentration and applying MTT method to detect OD value,and then observing effect of panax notoginseng saponing on activity.Results:(1) Panax notoginseng saponins can enhance activity of hippocampal neural stem cells under a certain concentration extent.(2) Hippocampal neural stem cells can differentiate into neuron and glial with panax notoginseng saponing.Conclusion: Panax notoginseng saponing can enhance the activity of hippocampus and promote hippocampus the differentiation of neural stem cells.