A Novel Fluorescence Probe for the Reversible Detection of Bisulfite and Hydrogen Peroxide Pair in Vitro and in Vivo |
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Authors: | Dr. Yue Wang Fang Zhou Prof. Dr. Qingtao Meng Songhe Zhang Dr. Hongmin Jia Dr. Cuiping Wang Dr. Run Zhang Prof. Dr. Zhiqiang Zhang |
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Affiliation: | 1. School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051 P. R. China;2. Anshan Tumor Hospital, 339 Shenhua Road, Lishan District, Anshan, Liaoning Province, P. R. China;3. Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051 P. R. China;4. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, 4072 Australia |
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Abstract: | The detection of changes in the reactive oxygen species (ROS)/reactive sulfur species (RSS) couple is important for studying the cellular redox state. Herein, we developed a 1,8-naphthalimide-based fluorescence probe ( NI ) for the reversible detection of bisulfite (HSO3−) and hydrogen peroxide (H2O2) in vitro and in vivo. NI has been designed with a reactive ethylene unit which specifically reacts with HSO3− by a Michael addition reaction mechanism, resulting in the quenching of yellow fluorescence at 580 nm and the appearing of green fluorescence at 510 nm upon excitation at 500 nm and 430 nm, respectively. The addition product ( NI−HSO3 ) could be specifically oxidized to form the original C=C bond of NI , recovering the fluorescence emission and color. The detection limits of NI for HSO3− and NI−HSO3 for H2O2 were calculated to be 2.05 μM and 4.23 μM, respectively. The reversible fluorescence response of NI towards HSO3−/H2O2 couple can be repeated for at least five times. NI is reliable at a broad pH range (pH 3.0–11.5) and features outstanding selectivity, which enabled its practical applications in biological and food samples. Monitoring the reversible and dynamic inter-conversion between HSO3− and H2O2 in vitro and in vivo has been verified by fluorescence imaging in live HeLa cells, adult zebrafish and nude mice. Moreover, NI has been successfully applied to detect of HSO3− levels in food samples. |
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Keywords: | fluorescence probe bisulfite hydrogen peroxide reversible bioimaging |
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