Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film |
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Authors: | Wei Zhang Tao Yang Da Ming Huang Kui Jiao |
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Institution: | College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China |
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Abstract: | A novel electrochemical DNA biosensor based on zinc oxide (ZnO) nanoparticles and multi-walled carbon nanotubes (MWNTs)for DNA immobilization and enhanced hybridization detection is presented. The MWNTs/nano ZnO/chitosan composite filmmodified glassy carbon electrode (MWNTs/ZnO/CHIT/GCE) was fabricated and DNA probes were immobilized on the electrodesurface. The hybridization events were monitored by differential pulse voltammetry (DPV) using methylene blue (MB) as anindicator. The sensor can effectively discriminate different DNA sequences related to PAT gene in the.transgenic corn, with adetection limit of 2.8×10-12 mol/L of target sequence.2008 Kui Jiao. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved. |
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Keywords: | ZnO nanoparticles Multi-walled carbon nanotubes Chitosan DNA sensor PAT gene |
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