Separation and determination of flavone and xanthone glycosides in Tibetan folk medicinal species Swertia mussotii and S. franchetiana by capillary electrophoresis |
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Authors: | Y -L Li C -X Ding H -L Wang Y -R Suo J -M You G -C Chen |
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Institution: | (1) Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, 810001, China;(2) Department of Chemistry, Qufu Normal University, Qufu, Shandong, 273165, China;(3) Graduate University of the Chinese Academy of Sciences, Beijing, 100039, China |
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Abstract: | The contents of five pharmacologically active flavone and xanthone glycosides, namely, swertianolin, swertisin, isoorientin,
mangiferin, and 7-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-xylopyranosyl]-1,8-dihydroxy-3-methoxyxanthone, extracted from Tibetan
folk medicinal species Swertia mussotii and S. franchetiana were determined by capillary electrophoresis with diode-array detection. The separation of five components has been optimized
with a capillary column with a total length of 48.5 cm and effective length of 40 cm (50 μm i.d). The influence of the running
buffer, the sodium dodecyl sulfonate (SDS) concentration, organic modifier, etc. on the resolution was evaluated. The background electrolyte contained 30 mM borate
buffer, 28 mM SDS, 1.0% (v/v) acetonitrile, and was adjusted to pH 9.0 with 0.1 M NaOH. A good baseline resolution was obtained
for the separation of five components within 5 min with the working voltage of 24 kV and a column temperature of 25°C. The
established method was rapid and reproducible for the separation and determination of five flavone and xanthone glycosides
from the extracts of S. mussotii and franchetiana plant samples.
The text was submitted by the authors in English. |
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