| 去乙酰化酶抑制剂TSA对间充质干细胞C3H10T1/2增殖和成脂分化的影响 |
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| 引用本文： | 吴伟,季菊玲,王奎标,李智耀,龚雨琴,季煜华.去乙酰化酶抑制剂TSA对间充质干细胞C3H10T1/2增殖和成脂分化的影响[J].暨南大学学报,2011,32(3). |
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| 作者姓名： | 吴伟 季菊玲 王奎标 李智耀 龚雨琴 季煜华 |
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| 作者单位： | 1. 暨南大学生命科学技术学院组织移植与免疫实验中心,广东广州,510632
2. 南通大学医学院病理教研室,江苏南通,226001 |
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| 基金项目： | 国家自然科学基金项目(30600587;3090056;381071483); 中央高校基本科研业务费专项资金项目; 江苏省教育厅自然科学基金(10KJB180006); 南通市科技局项目(K2009028); 南通大学自然科学项目(07Z089) |
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| 摘 要： | 采用MTT和流式细胞术分别检测不同浓度的TSA对C3H10T1/2细胞活性和细胞周期分布的影响;油红O染色检测TSA对其成脂分化的影响,实时定量PCR检测TSA对成脂分化的关键转录因子PPAR-γ,以及成脂分化标志物Fabp4和Adipoq mRNA转录的影响.研究去乙酰化酶抑制剂TSA对间充质干细胞C3H10T1/2增殖和成脂分化的影响及其可能的作用机制.结果显示TSA浓度为1、10和30 nmol/L呈浓度依赖性地抑制C3H10T1/2细胞活性,改变细胞形态,并将其细胞周期抑制在G0/G1期;TSA浓度为10nmol/L明显抑制C3H10T1/2细胞的成脂分化作用,并呈浓度依赖性地抑制PPAR-γ、Fabp4和Adipoq mRNA的转录.表明TSA呈剂量依赖性地抑制间充质干细胞C3H10T1/2的增殖和成脂分化,除转录水平调控外,非组蛋白如细胞骨架相关蛋白可能也参与TSA的抑制作用.
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| 关 键 词： | 曲古抑菌素A 间充质干细胞 成脂分化 增殖 |
Effects of histone deacetylase inhibitor trichostatin A on proliferation and adipogenic differentiation of C3H10T1/2 mesenchymal stem cells |
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| WU Wei,JI Ju-ling,WANG Kui-dong,LI Zhi-yao,GONG Yu-qin,JI Yu-hua.Effects of histone deacetylase inhibitor trichostatin A on proliferation and adipogenic differentiation of C3H10T1/2 mesenchymal stem cells[J].Journal of Jinan University(Natural Science & Medicine Edition),2011,32(3). |
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| Authors: | WU Wei JI Ju-ling WANG Kui-dong LI Zhi-yao GONG Yu-qin JI Yu-hua |
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| Institution: | WU Wei1,JI Ju-ling2,WANG Kui-dong1,LI Zhi-yao1,GONG Yu-qin1,JI Yu-hua1(1.Institute of Tissue Transplantation and Immunology,College of Life Science and Technology,Jinan University,Guangzhou 510632,China,2.Department of Pathology,College of Medicine,Jiangsu Nantong University,JiangsuNantong 226001,) |
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| Abstract: | To investigate the effects of histone deactylase(HDA) inhibitor trichostatin A on proliferation and adipogenic differentiation of C3H10T1/2 mesenchymal stem cells as well as the underlying mechanism.MTT assay and flow cytometry-based cell cycle analysis were used to evaluate the effects of trichostatin(TSA) at different concentrations on cell viability and cell cycle of cultured C3H10T1/2 cells.The influence of serial concentrations of TSA on adipogenic differentiation of C3H10T1/2 cells was determined by o... |
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| Keywords: | TSA mesenchymal stem cells adipogenic differentiation proliferation |
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