| 以乙二胺为手臂分子制备的DNA修饰电极及其伏安性能 |
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| 引用本文： | 焦奎,徐桂云,张旭志,米春华. 以乙二胺为手臂分子制备的DNA修饰电极及其伏安性能[J]. 高等学校化学学报, 2005, 26(5): 841-843 |
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| 作者姓名： | 焦奎 徐桂云 张旭志 米春华 |
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| 作者单位： | 1. 青岛科技大学化学与分子工程学院, 青岛266042;2. 中国海洋大学化学与化工学院, 青岛266003 |
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| 摘 要： | Carboxyl was formed on the surface of glassy carbon electrode(GCE) by electrochemical oxidation. Ethylenediamine(En) was used as the arm molecule to link carboxyl with dsDNA using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N- hydroxysuccinimide (NHS) as the activators to prepare dsDNA modified electrode(dsDNA/En/GCE). It was shown that dsDNA couM be covalently immobilized on the surface of GCE. ssDNA modified electrode(ssDNA/En/GCE) was obtained via the thermal denaturation of dsDNA/En/GCE. The dsDNA/En/GCE and ssDNA/En/GCE were characterized by voltammetry with methylene blue(MB) as the indicator. The results indicated that the currents of the redox peaks of MB at ssDNA/En/GCE were larger than those at dsDNA/En/GCE, and the currents of the redox peaks at En/GCE were the smallest. The peak-currents of MB at the DNA modified electrode had good reproducibility after multi-denaturation and hybridization cycles.
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| 关 键 词： | 脱氧核糖核酸(DNA) 乙二胺 共价固定 伏安法 亚甲基蓝 |
| 文章编号： | 0251-0790(2005)05-0841-03 |
| 收稿时间： | 2004-06-23 |
Preparation and Voltammetric Characterization of DNA Modified Electrode with Ethylenediamine as Arm Molecule |
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| JIAO Kui,XU gui-yun,ZHANG Xu-Zhi,MI Chun-Hua. Preparation and Voltammetric Characterization of DNA Modified Electrode with Ethylenediamine as Arm Molecule[J]. Chemical Research In Chinese Universities, 2005, 26(5): 841-843 |
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| Authors: | JIAO Kui XU gui-yun ZHANG Xu-Zhi MI Chun-Hua |
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| Affiliation: | 1. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;2. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Carboxyl was formed on the surface of glassy carbon electrode(GCE) by electrochemical oxidation. Ethylenediamine(En) was used as the arm molecule to link carboxyl with dsDNA using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS) as the activators to prepare dsDNA modified electrode(dsDNA/En/GCE). It was shown that dsDNA could be covalently immobilized on the surface of GCE. ssDNA modified electrode(ssDNA/En/GCE) was obtained via the thermal denaturation of dsDNA/En/GCE. The dsDNA/En/GCE and ssDNA/En/GCE were characterized by voltammetry with methylene blue(MB) as the indicator. The results indicated that the currents of the redox peaks of MB at ssDNA/En/GCE were larger than those at dsDNA/En/GCE, and the currents of the redox peaks at En/GCE were the smallest. The peak-currents of MB at the DNA modified electrode had good reproducibility after multi-denaturation and hybridization cycles. |
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| Keywords: | DNA Ethylenediamine Covalent immobilization Voltammetry Methylene blue |
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