Imaging of EdU, an alkyne-tagged cell proliferation probe, by Raman microscopy |
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| Authors: | Yamakoshi Hiroyuki Dodo Kosuke Okada Masaya Ando Jun Palonpon Almar Fujita Katsumasa Kawata Satoshi Sodeoka Mikiko |
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| Institution: | Sodeoka Live Cell Chemistry Project, ERATO, Japan Science and Technology Agency, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. |
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| Abstract: | Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules. |
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