Facile preparation of hydrophilic glutathione modified magnetic nanomaterials for specific enrichment of glycopeptides |
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| Institution: | 1. College of Chemistry, Jilin University, Changchun 130012, China;2. The Second Hospital of Jilin University, Changchun 130041, China;3. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China;1. Department of Chemistry, Fudan University, Shanghai 200433, China;2. Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China;1. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China;2. State Key Laboratory of Proteomics, National Center for Protein Science, Beijing 102200, China;3. National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China;1. School of Pharmacy, East-China University of Science and Technology, Shanghai 200237, China;2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;1. Phase I Clinical Trial Center, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, PR China;2. State Key Laboratory of Proteomics, National Center for Protein Sciences Beijing, Beijing Institute of Lifeomics, Beijing Proteome Research Center, Beijing 102206, PR China;3. Beijing Institute of Metrologe, Beijing 100022, PR China;1. Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai 200032, China;2. Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China;3. Department of Chemistry, Fudan University, Shanghai 200433, China |
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| Abstract: | Investigations of glycosylated proteins or peptides and their related biological pathways provide new possibilities for illuminating the physiological and pathological mechanisms of glycosylation modification. However, open-ended and in-depth analysis of glycoproteomics is usually subjected to the low-abundance of glycopeptides, heterogeneous glycans, and a variety of interference molecules. In order to alleviate the influence of these obstacles, effective preconcentration of glycopeptides are indispensable. Here, we employed a hydrophilic interaction liquid chromatography (HILIC)-based method to universally capture glycopeptides. Glutathione modified magnetic nanoparticles (Fe3O4@Au-GSH) were synthesized through a simple process and exploited to enrich glycopeptides from complex samples. The prepared materials showed excellent ability to trap glycopeptides from standard glycoproteins digests, low detection limit (10 fmol/μL), and good selectivity (HRP:BSA = 1:100). These results indicated that glutathione-based magnetic nanoparticles synthesized in this work had great potential for glycopeptides enrichment. |
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| Keywords: | Hydrophilic Glutathione Magnetic Enrichment Glycopeptides |
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