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Simultaneous Dual Encoding of Three‐Dimensional Structures by Light‐Induced Modular Ligation |
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| Authors: | Tanja K Claus Dr Benjamin Richter Vincent Hahn Dr Alexander Welle Sven Kayser Prof?Dr Martin Wegener Prof?Dr Martin Bastmeyer Dr Guillaume Delaittre Prof?Dr Christopher Barner‐Kowollik |
| Institution: | 1. Preparative Macromolecular Chemistry, Institut für Technische Chemie und Polymerchemie, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany;2. Institut für Biologische Grenzfl?chen (IBG), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany;3. Cell- and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany;4. Institute of Applied Physics and Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany;5. ION-TOF GmbH, Münster, Germany;6. Institut für Funktionelle Grenzfl?chen (IFG), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany;7. Institute for Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany;8. http://www.macroarc.de |
| Abstract: | A highly efficient strategy for the simultaneous dual surface encoding of 2D and 3D microscaffolds is reported. The combination of an oligo(ethylene glycol)‐based network with two novel and readily synthesized monomers with photoreactive side chains yields two new photoresists, which can be used for the fabrication of microstructures (by two‐photon polymerization) that exhibit a dual‐photoreactive surface. By combining both functional photoresists into one scaffold, a dual functionalization pattern can be obtained by a single irradiation step in the presence of adequate reaction partners based on a self‐sorting mechanism. The versatility of the approach is shown by the dual patterning of halogenated and fluorescent markers as well as proteins. Furthermore, we introduce a new ToF–SIMS mode (“delayed extraction”) for the characterization of the obtained microstructures that combines high mass resolution with improved lateral resolution. |
| Keywords: | cycloaddition direct laser writing microstructures patterning photochemistry |