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Solid‐State NMR of PEGylated Proteins |
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| Authors: | Dr. Enrico Ravera Silvia Ciambellotti Dr. Linda Cerofolini Dr. Tommaso Martelli Dr. Tatiana Kozyreva Dr. Caterina Bernacchioni Stefano Giuntini Prof. Marco Fragai Prof. Paola Turano Prof. Claudio Luchinat |
| Affiliation: | 1. Magnetic Resonance Center (CERM) and Department of Chemistry “Ugo Schiff”, University of Florence, and Magnetic Resonance Consortium (CIRMMP), Sesto Fiorentino, FI, Italy;2. Giotto Biotech S.r.l. Via Madonna del Piano 6, Sesto Fiorentino, FI, Italy |
| Abstract: | PEGylated proteins are widely used in biomedicine but, in spite of their importance, no atomic‐level information is available since they are generally resistant to structural characterization approaches. PEGylated proteins are shown here to yield highly resolved solid‐state NMR spectra, which allows assessment of the structural integrity of proteins when PEGylated for therapeutic or diagnostic use. |
| Keywords: | biopharmaceuticals PEGylated proteins protein modification protein structures solid-state NMR |