Imaging Glycosylation In Vivo by Metabolic Labeling and Magnetic Resonance Imaging |
| |
| Authors: | Dr André A Neves Dr Yéléna A Wainman Dr Alan Wright Dr Mikko I Kettunen Dr Tiago B Rodrigues Sarah McGuire Dr De‐En Hu Flaviu Bulat Dr Simonetta Geninatti?Crich Dr Henning Stöckmann Dr Finian J Leeper Prof Kevin M Brindle |
| |
| Institution: | 1. Cancer Research UK Cambridge Institute, Li Ka Shing Centre, Cambridge, UK;2. Department of Chemistry, University of Cambridge, Cambridge, UK;3. A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland;4. Department of Molecular Biotechnology and Health Science, Molecular Imaging Center, Turin, Italy |
| |
| Abstract: | Glycosylation is a ubiquitous post‐translational modification, present in over 50 % of the proteins in the human genome, 1 with important roles in cell–cell communication and migration. Interest in glycome profiling has increased with the realization that glycans can be used as biomarkers of many diseases, 2 including cancer. 3 We report here the first tomographic imaging of glycosylated tissues in live mice by using metabolic labeling and a gadolinium‐based bioorthogonal MRI probe. Significant N‐azidoacetylgalactosamine dependent T1 contrast was observed in vivo two hours after probe administration. Tumor, kidney, and liver showed significant contrast, and several other tissues, including the pancreas, spleen, heart, and intestines, showed a very high contrast (>10‐fold). This approach has the potential to enable the rapid and non‐invasive magnetic resonance imaging of glycosylated tissues in vivo in preclinical models of disease. |
| |
| Keywords: | bioorthogonal chemistry cancer gadolinium glycans magnetic resonance imaging |
|
|
