Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy |
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Authors: | Jonathan B. Grimm Dr. Teresa Klein Dr. Benjamin G. Kopek Dr. Gleb Shtengel Dr. Harald F. Hess Prof. Dr. Markus Sauer Dr. Luke D. Lavis |
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Affiliation: | 1. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA;2. Department of Biotechnology and Biophysics, Julius Maximilian University Wuerzburg, Wuerzburg, Germany;3. Hope College, Department of Biology, Holland, MI, USA |
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Abstract: | The rhodamine system is a flexible framework for building small‐molecule fluorescent probes. Changing N‐substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si‐containing analogue of Q‐rhodamine. This probe is the first example of a “caged” Si‐rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red‐shifted to allow multicolor imaging. The dye is a useful label for super‐resolution imaging and constitutes a new scaffold for far‐red fluorogenic molecules. |
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Keywords: | fluorophore microscopy photoactivation Si-rhodamine super-resolution imaging |
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