Discerning the Chemistry in Individual Organelles with Small‐Molecule Fluorescent Probes |
| |
| Authors: | Dr Wang Xu Prof Dr Zebing Zeng Prof Dr Jian‐Hui Jiang Prof Dr Young‐Tae Chang Prof Dr Lin Yuan |
| |
| Institution: | 1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P.R. China;2. Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, Singapore;3. Laboratory of Bioimaging Probe Development, A*STAR, Singapore;4. Department of Chemistry, Stanford University, USA |
| |
| Abstract: | Principle has it that even the most advanced super‐resolution microscope would be futile in providing biological insight into subcellular matrices without well‐designed fluorescent tags/probes. Developments in biology have increasingly been boosted by advances of chemistry, with one prominent example being small‐molecule fluorescent probes that not only allow cellular‐level imaging, but also subcellular imaging. A majority, if not all, of the chemical/biological events take place inside cellular organelles, and researchers have been shifting their attention towards these substructures with the help of fluorescence techniques. This Review summarizes the existing fluorescent probes that target chemical/biological events within a single organelle. More importantly, organelle‐anchoring strategies are described and emphasized to inspire the design of new generations of fluorescent probes, before concluding with future prospects on the possible further development of chemical biology. |
| |
| Keywords: | anchoring motif cell organelles cellular analytes fluorescence imaging fluorescent probes |
|
|
