氧化铁磁性纳米粒子固定化酶

纳米粒子作为酶固定化的载体,当其具有磁性时,制备的固定化酶易于从反应体系中分离和回收,操作简便;并且利用外部磁场可以控制磁性材料固定化酶的运动方式和方向,替代传统的机械搅拌方式,提高固定化酶的催化效率。在众多纳米材料中,氧化铁因其在磁性、催化等多方面的良好特性而倍受瞩目。本文对近年来各种氧化铁磁性纳米粒子固定化酶,尤其是固定化脂肪酶和蛋白酶的制备方法及其应用做了较为详细的阐述,对这些氧化铁磁性纳米粒子固定化酶的优缺点和发展前景进行了讨论。

Magnetic Iron Oxide Nanoparticles Immobilized Enzymes

Nanoparticles can be used as the carriers of immobilized enzymes,and the magnetic nanoparticle immobilized enzymes are easy to operate, separate and recover from the reaction system. Moreover, utilizing an external magnetic field the movement manner and direction of the immobilized enzyme can be controlled to improve their catalytic efficiency, compared with the traditional mechanical stirring ways. Among many nano-materials, iron oxide has received considerable attention because of its magnetic, catalytic, and other good characteristics of high-profile. In this paper, a variety of magnetic iron oxide nanoparticles immobilized enzymes, especially the preparation and application of immobilized lipase and protease are reviewed. The advantages and shortcomings of the magnetic iron oxide nanoparticles immobilized enzymes and the future developing prospects are also discussed.Contents
1 Introduction
2 Magnetic iron oxide nanoparticles immobilized lipase
2.1 Magnetic polymer microsphere immobilized lipase
2.2 Magnetic nanoparticles directly immobilized lipase
2.3 Magnetic siliceous mesocellular foam immobilized lipase
3 Magnetic iron oxide nanoparticles immobilized protease
3.1 Immobilized α-chymotrypsin
3.2 Immobilized papain
3.3 Immobilized trypsin
4 Other magnetic iron oxide nanoparticles immobilized enzymes
5 Conclusion and outlook