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| Authors: | N Kaushik A Agnihotri |
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| Institution: | (1) Biotechnology Division, Tata Energy Research Institute, Darbari Seth Block, Habitat Place, Lodi Road, 110 003 New Delhi, India |
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| Abstract: | Summary An efficient and economical GC method for rapid determination of FAMEs in rapeseed-mustard is described. The seeds were transmethylated
with acetylchloride, using microwave heating and separation achieved on a 3 m column packed with a mixture of 2% SP-2300 and
3% SP-2310 on Chromosorb ‘W’. The method is compared with the conventional heating method and extended efficiently for half-seed
analysis. The fatty acid composition of the FAMEs mixtures prepared by both methods was similar with highly significant correlation
coefficients (P<0.001). |
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| Keywords: | Gas chromatography FAMEs Rapeseed-mustard Half-seeds |
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