Quantitative determination of glycine in commercial dosage forms by kinetic spectrophotometry |
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| Authors: | Snežana S Mitić Aleksandra N Pavlović Snežana B Tošić Biljana B Arsić Slavica M Sunarić |
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| Institution: | (1) Faculty of Sciences and Mathematics, Department of Chemistry, University of Niš, Višegradska 33, P.O. Box 224, 18000 Niš, Serbia;(2) Faculty of Medical Sciences, Bulevar Dr Zorana Dindica 81, 18000 Niš, Serbia |
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| Abstract: | A kinetic method for the determination of nanogram quantities of amino acid glycine (Gly) is described. The catalytic activity
of cobalt in the reaction of oxidation of purpurin (1,2,4-trihydroxyantraquinone, PP) by hydrogen peroxide in alkaline buffer
solution decreases in the presence of micro quantities of glycine. Operating conditions for the successful determination of
glycine were optimized and yielded a theoretical detection limit of 6.5 ng/mL. Kinetic equations are proposed for the investigated
process. The interference effects of certain foreign ions and amino acids upon the reaction rate were studied in order to
assess the selectivity of the method. The procedure was successfully applied to the determination of glycine in various pharmaceutical
samples. The unique features of this procedure are that the determination can be carried out rapidly at room temperature and
analysis time is short. The procedure is simple, inexpensive and efficient for use in the analysis of a large number of samples.
The article is published in the original. |
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