Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip |
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| Authors: | Nagatani Naoki Yamanaka Keiichiro Ushijima Hiromi Koketsu Ritsuko Sasaki Tadahiro Ikuta Kazuyoshi Saito Masato Miyahara Toshiro Tamiya Eiichi |
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| Institution: | Department of Applied Chemistry, Graduate School of Engineering, Okayama University of Science, Okayama-shi 700-0005, Japan. nagatani@dac.ous.ac.jp |
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| Abstract: | Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min. |
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