基于光线传感器的手机光度计设计与应用

利用智能手机的光线传感器设计了一款简易光度计,并为其开发了安卓手机应用,可以在线查看样品信号,自动计算吸光度值。此款简易光度计采用了液相色谱紫外检测器的流通池作为样品吸收池,利用光纤传导光信号,可以大幅降低试剂使用量。进行一次测试只需要使用约200μL样品,有效降低了实验室废液回收量。利用该光度计对铁离子标准溶液进行测定,在测定浓度范围内,吸光度与样品浓度具有良好的线性拟合关系,其中R2=0.9991,可取代化学实验中的传统分光光度计。利用该光度计进行实验可以强化学生对仪器和实验原理的理解,有利于培养创新意识。

Design and Application of Mobile-Phone Photometer Based on Light Sensor

By using the light sensor of smartphone, a simple photometer was designed. Also, an Android APP was developed to show the online signal and calculate the absorbance automatically. This photometer uses a flow cell of HPLC UV-detector as sample absorption cell and an optical fiber to transfer the light signal. The mobile-phone photometer can greatly reduce the amount of reagents used. Only 200 μL sample is needed to run a test, which effectively reduces laboratory waste. There is a good linear relationship with R² value 0.9991 while using the photometer to measure the iron ion standard solution, which means it can replace the traditional spectrophotometer in chemical experiments. Students can enhance their understanding of the instrument principles and inspire their creativity by using this DIY photometer.