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致病性鸡大肠杆菌噬菌体的分离与效价测定
引用本文:孙海虹,马同锁,于珊,赵宝华.致病性鸡大肠杆菌噬菌体的分离与效价测定[J].河北师范大学学报(自然科学版),2007,31(1):108-111,115.
作者姓名:孙海虹  马同锁  于珊  赵宝华
作者单位:1. 河北师范大学,生命科学学院,河北,石家庄,050016
2. 河北经贸大学,生物科学与工程学院,河北,石家庄,050061
基金项目:河北师范大学校科研和教改项目
摘    要:鉴于临床分离的致病性大肠杆菌的耐药谱很广,常规抗生素的治疗效果较差,死疫苗往往存在毒力返祖的问题.实验从污水中分离得到了5株噬菌体,采用自行分离和鉴定的大肠杆菌为宿主菌,经纯化增殖提高效价后进行裂解试验,取得了比较满意的效果.

关 键 词:鸡大肠杆菌  噬菌体  裂解
文章编号:1000-5854(2007)01-0108-04
修稿时间:2006-09-252006-11-19

Phage Isolates and Lysis of Chicken Escherichia coli
SUN Hai-hong,MA Tong-suo,YU Shan,ZHAO Bao-hua.Phage Isolates and Lysis of Chicken Escherichia coli[J].Journal of Hebei Normal University,2007,31(1):108-111,115.
Authors:SUN Hai-hong  MA Tong-suo  YU Shan  ZHAO Bao-hua
Institution:1. College of Life Scienee,Hebei Normal University, Hebei Shijiazhuang 050016 ,China; 2. College of Biology Scienee and Bioteehnology,Hebei University of Economies and Trade,Hebei Shijiazhuang 050061 ,China
Abstract:The antimicrobial-resistant strains of Escherichia coli separated from clinic is very wide.Experiment shows that average resistant ratio of E.coli to eighteen kinds of antibacterial agents is nearly 80 percent.Common antibacterial agents'therapy effects are usually far from satisfaction.However,some phages obtained from sewage by purifying and propagating show better effects.The lysis ratios of 5 phages to A,B,C and D are very high.
Keywords:chicken colibacillosis  phage  lysis
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