532 nm激光诱导荧光毛细管阵列电泳仪的研制

基于共聚焦激光诱导荧光检测技术,研制了一台旋转扫描高效毛细管阵列电泳装置.以波长为532nm的半导体二极管激光器作为激发光源,多根毛细管阵列采用圆形布局,微型高速直流电机带动旋转反射镜进行激光扫描,加快了数据采集的速度;采用旋转编码器实现了毛细管的定位与位置读出,并触发数据采集系统进行荧光信号采集.以罗丹明6G和罗丹明B为分析样品,考察了16通道毛细管阵列电泳仪的基本性能.

Design and Evaluation of Capillary Array Electrophoresis with 532 nm Laser-induced Fluorescence Detection

A high performance capillary array electrophoresis device with rotary scanner,based on confocal laser-induced fluorescence detection techniques has been developed.The miniature high speed direct current motor,along with a reflection mirror was introduced to accelerate the speed of data acquisition and direct the focused excitation laser beam with 532 nm on the detection window of capillaries,which were symmetrically fixed in the grooved cylinder ring.The Absolute rotary encoder was used to locate every capillary and trigger the data acquisition system to collect the fluorescence signal corresponding to the position of each capillary.The basic performance of 16-channel capillary array electrophoresis was evaluated by using Rhodamine 6G and Rhodamine B as test samples.