Binding evaluation of isoform 1 fromCratylia mollis lectin to human mammary tissues |
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| Authors: | Eduardo I C Beltrão Maria T S Correia José Figueredo-Silva Luana C B B Coelho |
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| Institution: | (1) Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, UFPE, 50670-490 PE, Brasil;(2) Departamento de Patologia-CCS, Universidade Federal de Pernambuco, UFPE, 50670-490 PE, Brasil;(3) Laboratório de Imunopatologia Keizo Asami, LIKA, Universidade Federal de Pernambuco, UFPE, 50670-490 PE, Brasil |
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| Abstract: | The phenomenon of altered carbohydrates in transformed cell surfaces has been studied through histochemical techniques using
lectins. Specific binding patterns to normal and transformed mammary tissues were evaluated by Isoform 1 fromCratylia mollis lectin (Cra Iso 1). Protocols using a direct method, incubation of Cra Iso I conjugated to peroxidase (Cra Iso 1-Per) with
mammary tissues, followed by diaminobenzidine and hydrogen peroxidase interaction, were performed. Neoplastic tissues, marked
by Cra Iso 1, showed a higher intensity of staining than normal ones, in comparison withCanavalia ensiformis lectin, Concanavalin A (Con A), conjugated to peroxidase (Con A-Per). The assay with Cra Iso 1 also indicated a possible
utilization of this lectin to characterize normal and transformed mammary cells. |
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| Keywords: | Lectin isoform human mammary tissue infiltrating duct carcinoma fibrocystic disease fibroadenoma |
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