电堆集富集非水毛细管电泳同时测定水杨酸、肉桂酸、阿魏酸和香草酸

建立了同时分离测定水杨酸、肉桂酸、阿魏酸和香草酸的电堆集富集-非水毛细管电泳(NACE)的新方法。运行缓冲溶液为40mmol/L乙酸钠-2.5mmol/L氢氧化钠甲醇溶液,电压-25kV,在225nm波长下紫外检测。对电压、乙酸钠浓度、氢氧化钠浓度、进样时间、样品溶液等因素对电堆集及分离的影响做了系统的研究。水杨酸、肉桂酸、阿魏酸和香草酸分别在1.4~28mg/L、0.40~8.0mg/L、0.7~18mg/L和0.7~30mg/L范围内线性关系良好(r=0.9999、r=0.9997、r=0.9994、r=0.9997);回收率分别为95.8~99.6%、96.2~98·2%、95.7~105%和98.9~103%,基于3倍信噪比(S/N=3),4种有机酸的检出限分别为0.069、0.051、0.107和0.089mg/L。

Separation and Determination of Salicylic Acid,Cinnamic Acid,Ferulic Acid and Vanillic Acid with Sample Stacking-Non-aqueous Electrophoresis

A new method for the simultaneous determination of salicylic acid, cinnamic acid, ferulic acid and vanillic acid by stacking-non-aquous-capillary electrophoresis has been proposed. The operating buffer was composed of 40 mmol/L sodium acetate -2.5 mmol/L sodium hydroxide in methanol.The applied voltage was -25 kV and the UV detection wavelength was 225 nm. The linear ranges of the method were 1.4-28 mg/L (r=0.9999) for salicylic acid, 0.40-8.0 mg/L (r=0.9997) for cinnamic acid, 0.7-18 mg/L (r=0.9994) for ferulic acid and 0.7-30 mg/L (r=0.9997) for vanillic acid, the recoveries were 95.8%-99.6%,96.2%-98.2%,95.7%-105%,98.9%-103% and detection limits (S/N=3) of 0.069, 0.051, 0.107 and 0.089 mg/L for 4 organic acids respectively.