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| 双柱吸附法对莲子心总碱的分离纯化研究 | |
| 引用本文： | 张吉祥,郭金生,杨佳玲,欧来良.双柱吸附法对莲子心总碱的分离纯化研究[J].离子交换与吸附,2008,24(6). |
| 作者姓名： | 张吉祥 郭金生 杨佳玲 欧来良 |
| 作者单位： | 1. 廊坊师范学院化学系,廊坊,065000 2. 天津市产品质量监督检测技术研究院,天津,300384 3. 南开大学生物活性材料教育部重点实验室,分子生物研究所,天津,300071 |
| 基金项目： | 河北省教育厅基金资助项目 |
| 摘 要： | 研究了大孔吸附树脂和阳离子交换树脂双柱吸附法对莲心碱、异莲心碱和甲基莲心碱的吸附行为。利用阳离子交换树脂D72制备出莲心碱、异莲心碱和甲基莲心碱,含量分别为10.6%、10.6%和28.0%,总含量为49.2%的莲子心总碱提取物,其中杂质主要是水溶性较大的生物碱成分。利用AKS-W大孔吸附树脂能去除极性较大的生物碱,而D72阳离子交换树脂能去除非生物碱的特点,采用双柱联合吸附法,制备出3种吸附质含量分别为33.1%、15.0%和34.5%,总含量为82.6%的莲子心总碱提取物。 |
| 关 键 词： | 双柱吸附法 大孔吸附树脂 阳离子交换树脂 莲子心总碱 |
ISOLATION AND PURIFICATION OF ALKALOIDS FROM PLUMULA NELUMBINIS BY DOUBLE-COLIUMN ADSORPTION CHROMATOGRAPHY |
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| ZHANG Jixiang,GUO Jinsheng,YANG Jialing,OU Lailiang.ISOLATION AND PURIFICATION OF ALKALOIDS FROM PLUMULA NELUMBINIS BY DOUBLE-COLIUMN ADSORPTION CHROMATOGRAPHY[J].Ion Exchange and Adsorption,2008,24(6). | |
| Authors: | ZHANG Jixiang GUO Jinsheng YANG Jialing OU Lailiang |
| Institution: | ZHANG Jixiang1 GUO Jinsheng1 YANG Jialing3 OU Lailiang2* 1. Department of Chemistry,Langfang Normal College,Langfang 065000,China 2. Key Laboratory of Bioactive Materials,Ministry of Education,Institute of Molecular Biology,Nankai University,Tianjin 300071,China 3. Tianjin Product Quality Inspection Technology Research Institute,Tianjin 300384 China |
| Abstract: | The performance of adsorption and separation for liensinine, isoliensinine and neferine was studied by double-column adsorption chromatography using macroporous adsorption and cation exchange resins. The alkaloid extract with 49.2% total contents by mass representing 10.6% liensinine, 10.6% isoliensinine and 28.0% neferine respectively was prepared by D72 cation exchange resins, in which most of the impurities were water-soluble alkaloids. Furthermore,the alkaloid extract with 82.6% total contents by mass containing 33.1%, 15.0% and 34.5% of the three adsorbates respectively was prepared by double-column adsorption chromatography using AKS-W macroporous adsorption and D72 cation exchange resins. As a result, the content of single and total alkaloids has been greatly increased by the double-column adsorption chromatography. |
| Keywords: | Double-column adsorption chromatography Macroporous adsorption resin Cation exchange resin Alkaloids from plumula nelumbinis |
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