A rapid in vitro assay of cobalamin in human urine and medical tablets using ICP-MS |
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| Authors: | Gai Zhang Jianbo Liu Zuchao Meng Xin Wang |
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| Institution: | (1) School of Materials and Chemical Engineering, Xi’an Technological University, Xi’an, 710032, China;(2) Department of Chemistry, Xianyang Normal University, Xianyang, 712000, Shannxi Province, China;(3) College of Chemistry & Chemical Engneering, Xi’an Petroleum University, Xi’an, 710065, China;(4) Validation Department, Xian-Janssen Pharmaceutical Ltd., 34 North Wanshou Road, Xi’an, 710043, China |
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| Abstract: | A rapid and simple as well as sensitive inductively coupled plasma mass spectrometry (ICP-MS) method for the determination
of cobalamin is described. Cobalamin in human urine and medicine tablet solutions was converted on-line into free cobalt ions
in acid medium, the cobalt ions were then detected by ICP-MS. Cobalamin was determined by measuring the increase of integral
counts per second intensity, which was linear over the cobalamin concentration range of 1.0 × 10−10 g mL−1 to 8.0 × 10−5 g mL−1, and the limit of detection was 0.05 ng mL−1 (3σ). At the pump rate of 30 rotations per minute, one analysis cycle of cobalamin, including sampling and washing, could be
accomplished in 0.5 min with the relative standard deviations of less than 5 %. The proposed procedure was applied successfully
in monitoring cobalamin in human urine without any pretreatment process and in rapid determination of cobalamin in multivitamin
tablets. |
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| Keywords: | ICP-MS vitamin B12 cobalamin medicine |
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