Fast turnaround bioanalysis in discovery and early clinical development |
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| Authors: | C A James M Breda E Frigerio J Long K Munesada |
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| Institution: | (1) Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, 6720 Szeged, Hungary;(2) Department of Chemistry, Iowa State University, Gilmann Hall, Ames, IA, USA;(3) Eenheid Organische Chemie, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium |
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| Abstract: | Summary The difect and indirect separation of enantiomers of secondary amino acids was studied by high-performance liquid chromatography.
Direct separation was by using a macrocyclic glycopeptide, teicoplanin or ristocetin A, as chiral stationary phase. Indirect
separation was via pre-column derivatization with (S)-N-(4-nitrophenoxycarbonyl)phenylalanine methoxyethyl ester (S)-NIFE] as a new chiral derivatizing agent. Both direct and indirect separations were performed by means of reversed-phase
HPLC. Conditions for separations were optimized. The methods described offer good enantioselectivity for synthetic chiral
imino acids.
Presented at Balaton Symposium '01 on High-Performance Separation Methods, Siófok, Hungary, September 2–4, 2001 |
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| Keywords: | Column liquid chromatography Chirobiotic T and Chirobiotic R columns Imino acids Macrocyclic glycopeptides (S)-N-(4-Nitrophenoxycarbonyl)phenylalanine methoxyethyl ester (S)-NIFE |
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